Journal: Frontiers in Synaptic Neuroscience
Article Title: Different Synaptic Plasticity After Physiological and Psychological Stress in the Anterior Insular Cortex in an Observational Fear Mouse Model
doi: 10.3389/fnsyn.2022.851015
Figure Lengend Snippet: Changed presynaptic transmitter release probably within aIC of observer and demonstrator mice. (A) Left: schematic diagram showing location of the MED64 probe on the coronal IC slice; Right: light microscopy photograph showing relative location of aIC within the probe; (B) Spatial distribution of extracellular field potential induced by electrical stimulation on channel 45 (marked as red circle) in layers VI of aIC; (C,D) Input–output curve of fEPSP slope (%) vs. stimulus intensity (mA) in the slice among naïve ( n = 4 slices of 3 mice), observer ( n = 4 slices of 3 mice), and demonstrator ( n = 7 slices of 5 mice) in the superficial (C) and in the deep layers (D) of aIC; (E,F) Example traces of paired-pulse facilitation (PPF) with an interval of 25 ms recorded in the superficial (E) and deep layers (F) of aIC; (G,H) The paired-pulse ratios (slope of fEPSP2/slope of fEPSP1) recorded with intervals of 25, 50, 75, and 100 ms in superficial (G) and deep layers (H) of aIC; Data are shown as mean ± SEM (PPF: Naïve n = 4 slices of 3 mice, observer n = 7 slices of 6 mice, and demonstrator n = 4 slices of 4 mice) and compared by two-way ANOVA analysis followed by Tukey’s multiple comparisons test, * p < 0.05, vs. naïve.
Article Snippet: The 64-channel multielectrode array recording system (MED64; Panasonic Alpha-Med Sciences, Osaka, Japan) was used for extracellular field potential recordings.
Techniques: Light Microscopy